FIGURE 2.

(A) Schematic diagram illustrating the different layers of the microfabricated array: glass coverslip (1), gold electrodes (2), PDMS microchannel (3), and mineral oil (4). (B) A micrograph of an array of adult ventricular myocytes aligned to the microelectrodes by means of the PDMS microchannels. The field of view shown could be illuminated with a light source for fluorophore excitation and imaged with a fast low light camera through a high NA (0.9) 20× lens. This format thus has the potential to record Ca²⁺ transients from all the cells in the array simultaneously.