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Hyaluronan “footprints” of chondrocytes are left after application of shear stress. Chondrocytes were treated with flow as described in Fig. 5, then fixed and incubated with biotinylated hyaluronan binding proteins followed by incubation with strepavidin-CY3. Tracks of hyaluronan are visible upstream to the cells. Cell with hyaluronan “footprints” of 75 μm. The arrow marks the flow direction. Scale bar, 10 μm.
