TABLE 5.

The effect of mutations of positively charged residues on cytochrome f on its interactions with plastocyanin at 300 mm ionic strength

Cyt f	Successes/1000 Trajectories*	% Inhibition†	Rate of electron transport 10−8 (M−1 s−1)
WT	17.1 ± 4.0	—	5.2 ± 0.9
K189A	11.5 ± 3.8	33	3.5 ± 0.7
K65A	11.4 ± 3.4	33	3.5 ± 0.7
K189E	7.4 ± 0.5	57	2.3 ± 0.6
K65E	8.3 ± 0.7	51	2.6 ± 0.5
K58Q + K65S + K66A	8.9 ± 0.6	48	2.8 ± 0.6
K58Q + K65S + K66E + K188N + K189Q	5.4 ± 0.9	68	1.7 ± 0.6
Electrostatic field off	3.7 ± 0.1	78	1.1 ± 0.5

^*To obtain better statistics, five runs of 10,000 trajectories each were carried out at an ionic strength of 300 mM after which the number of successful trajectories with minimum Cu-Fe distance ≤20 Å were calculated. Other conditions were as for Table 3.

^†Compared to WT control at 300 mM.