TABLE 2.
Expt no. | % of DC that had bound or internalized C. neoformansa
|
||||
---|---|---|---|---|---|
IgG control | Anti-MR | Anti-CD64 | Anti-CD32 | Anti-CD16 | |
1 | 31 | 23 | 35 | 18 | 47 |
2 | 71 | 29 | 63 | 35 | 63 |
5 × 105 DC were incubated with 5 × 106 killed C. neoformans strain 67 cells in 24-well plates with 10 μg of antibody/ml for 18 h and then analyzed by light microscopy after Giemsa staining. Two hundred cells were counted. IgG, immunoglobulin G; MR, mannose receptor.