TABLE 1.
Translocation of nuclear transport factors NXT1 and NTF2 and “inert” molecules of similar size through the nuclear pore complex of Xenopus oocytes
| Substance | Molecular mass of monomer (kD) | Concentration in transport measurements μM of monomers | Import (I) Export (E) | Rate constant from exponential fit* (10−3 s−1) | Rate constant from deconvolution* (10−3 s−1) | Mean permeability P* (μm s−1) | Transport rate at actually used concentration† (molecules s−1 NPC−1) | Mean transport rate at ΔC = 1 μM† (molecules s−1 NPC−1) |
|---|---|---|---|---|---|---|---|---|
| NXT1 | 15 | 2.0 | I | 37.8 ± 3.43 (15) | 90.2 ± 15.4 (12) | 4.38 ± 0.470 (23) | 108.6 | 52.8 |
| E | 40.1 ± 3.53 (14) | 84.8 ± 11.1 (11) | 102.2 | |||||
| NTF2 | 15 | 4.0 | I | 30.1 ± 3.51 (9) | 63.7 ± 14.1 (8) | 3.93 ± 0.672 (14) | 153.6 | 47.3 |
| E | 47.4 ± 6.81 (10) | 98.6 ± 24.2 (6) | 237.2 | |||||
| α-Lact | 14 | 9.0 | I | 6.71 ± 0.90 (8) | 6.33 ± 1.48 (8) | 0.418 ± 0.0758 (17) | 34.3 | 5.03 |
| E | 6.70 ± 0.80 (9) | 10.2 ± 2.47 (9) | 55.1 | |||||
| GFP | 29 | 4.0 | I | 2.14 ± 0.40 (7) | 1.91 ± 0.31 (6) | 0.0881 ± 0.0119 (14) | 4.6 | 1.06 |
| E | 1.46 ± 0.42 (9) | 1.65 ± 0.36 (8) | 4.0 |
*
Mean ± SE of (n) measurements.
†
Calculated from deconvolved rate constant and σ = 50 NPC μm−2.