FIGURE 3.

Accumulated all-points histogram of all records analyzed in the E. coli IOM bilayer at 25°C without cholesterol and AmB concentration of 100 μM. The solution used was 2 M KCl, 1mM CaCl₂, and 10 mM Hepes at pH = 8.0. The applied membrane potential was 200 mV. The baseline at 0 current is not shown. In this case channel type V does not appear, but this histogram is shown because it corresponds to those conditions where channels are less likely to appear according to the assumption of sterols requirement.