TABLE 6.
Effective probabilities and effective conductances as described in the text for E. coli bilayers and other phosphatidylcholine-based bilayers in the presence or absence of sterols and different temperatures
| Without cholesterol
|
30 mol% cholesterol
|
30 mol% ergosterol
|
||||
|---|---|---|---|---|---|---|
| Bilayer (T°C) | Effective probability (%) | Effective conductance [pS] | Effective probability (%) | Effective conductance [pS] | Effective probability (%) | Effective conductance [pS] |
| DMPC | ||||||
| (30) | 3.1 | 0.5 (0.2) | 4.3 | 0.3 (0.1) p > 0.1 | ||
| Egg lecithin | ||||||
| (25) | 2.1 | 0.4 (0.1) | 7.7 | 2.8 (0.6) p < 0.01 | 27.7 | 3.5 (1.5) p < 0.05 |
| (15) | 8.5 | 1.4 (0.4) p < 0.05 | ||||
| E. coli IOM | ||||||
| (25) | 6.6 | 1.6 (0.8) | 12.8 | 3.4 (1.2)p < 0.05 | ||
| (15) | 11.1 | 1.4 (0.5)p > 0.1 | ||||
The membrane potential and buffer used are those quoted in Table 3. P-values are also reported to validate the changes in these properties upon the addition of sterols and temperature decrement. IOM, inner and outer membrane of E. coli; (), standard deviation.