FIGURE 1.

Lysozyme chromatograms for V_inj = 20 μL and different injected concentrations (C_inj) as indicated next to each curve. The average concentrations 〈C_i〉 are 0.74 mg/mL (dash); 1.72 mg/mL (points); 2.20 mg/mL (dash and points); 2.68 mg/mL (long dash); 3.08 mg/mL (long dash and points). The vertical line marks the retention time for the most dilute sample (not shown). The retention time t_r is the time corresponding to the peak of the concentration profile (C_max) and increases with increasing concentration. The retention time of completely included molecules (the “total” volume) is marked as t_T and is caused by the buffer. The retention time of completed excluded molecules (the “dead” volume) was t₀ = 6.07 min (not shown). C_max and 〈C_i〉 are similar. Buffer: sodium acetate, 50 mM, pH 4.7.