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Semilogarithmic SAXS patterns curves of C. aestuarii Hc in the presence of increasing concentrations of GuHCl (A and B) or NaCl (C and D), as indicated. For sake of clarity, the SAXS traces are shifted along the y-axis. Protein samples: A and C, hexameric Hc in the holoform; B and D, CaeSS2 subunit in the holoform. Fitting curves are obtained with the global fitting analysis based on the thermodynamic model of association/unfolding processes.
