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. 2003 Oct;85(4):2711–2719. doi: 10.1016/s0006-3495(03)74694-3

TABLE 1.

Comparison of measurements in a capillary and a microscope slide by FCS and PCH analysis

Slide Capillary
(FCS) g (0) 0.57 0.60
ω0 (mm) 0.25 0.27
(PCH) ɛ (cpsm) 43,000 22,000
N 0.15 0.12

The same dye solution was measured in a quartz capillary and on a microscope coverslip under otherwise identical conditions. PCH and FCS analysis were used to recover the fluctuation amplitude g(0), the beam waist ω0, the molecular brightness ɛ and the average number of particles N for both measurements. The diffusion coefficient of the dye rhodamine 110 was fixed to a value of 300 μm2/s.