TABLE 1.
Slide | Capillary | ||
---|---|---|---|
(FCS) | g (0) | 0.57 | 0.60 |
ω0 (mm) | 0.25 | 0.27 | |
(PCH) | ɛ (cpsm) | 43,000 | 22,000 |
N | 0.15 | 0.12 |
The same dye solution was measured in a quartz capillary and on a microscope coverslip under otherwise identical conditions. PCH and FCS analysis were used to recover the fluctuation amplitude g(0), the beam waist ω0, the molecular brightness ɛ and the average number of particles N for both measurements. The diffusion coefficient of the dye rhodamine 110 was fixed to a value of 300 μm2/s.