TABLE 3.
Dose pattern of LOS antibody responses elicited after i.n. immunization with dLOS-CRM conjugate
| No. of immunization dosesa | Antibody class | GMb antibody titers as determined by ELISA
|
||||
|---|---|---|---|---|---|---|
| Nasal wash | Lung lavage fluid | Saliva | Fecal extract | Serum | ||
| 5 | IgA | 140 (52-373)**c | 90 (23-346)** | 6 (2-17) | 15 (5-42)** | 90 (23-346)* |
| IgG | 12 (5-32)* | 19 (3-103)* | 3 | 5 (3-9) | 112 (18-682)* | |
| IgM | 3 | 4 (2-7) | 3 | 3 | 19 (11-35)* | |
| 4 | IgA | 225 (62-812)** | 156 (49-493)** | 12 (4-36)* | 25 (7-92)** | 130 (42-404)** |
| IgG | 43 (9-198)** | 36 (8-155)** | 8 (3-25) | 8 (3-25) | 324 (64-1,634)** | |
| IgM | 3 | 4 (2-6) | 3 | 3 | 17 (7-43) | |
| 3 | IgA | 43 (10-194)** | 43 (10-194)** | 7 (3-17) | 9 (2-54) | 36 (10-130) |
| IgG | 11 (2-60) | 16 (2-120) | 3 | 7 (3-17) | 130 (13-1,256)* | |
| IgM | 3 | 3 | 3 | 3 | 14 (8-25) | |
| 2 | IgA | 14 (4-45)* | 12 (8-19)** | 5 (3-11) | 7 (4-12)* | 17 (9-32) |
| IgG | 4 (2-6) | 4 (2-6) | 4 (2-6) | 8 (3-19)∗ | 30 (15-60)* | |
| IgM | 3 | 3 | 3 | 4 (2-8) | 21 (12-37)* | |
| 1 (or 0) | IgA | 3 | 3 | 3 | 3 | 10 |
| IgG | 3 | 3 | 3 | 3 | 10 | |
| IgM | 3 | 3 | 3 | 3 | 10 | |
a
Mice were i.n. immunized with one to five doses at 1-week intervals with 10 μl of PBS containing a mixture of 5 μg of dLOS-CRM and 1 μg of CT or were kept as naïve mouse controls.
b
Geometric mean (± SD range) of data from six mice.
c
Statistical significance for comparison with naïve mouse group: *, P < 0.05; **, P < 0.01.