FIGURE 1.

Hydration numbers of proteins estimated using dielectric spectroscopy. The ordinate (N_total) is the number of hydrating water molecules per protein molecule calculated from the dielectric exclusion volume, and the abscissa (N_cal) is the solvent-accessible surface areas (ASA)-based estimate. Data were from Yokoyama et al. (2001) and Suzuki et al. (1997b), for which the line was drawn by a linear regression analysis excluding the unfilled circle denoting a value for F-actin on the basis of its monomer unit estimated in this study (see Table 1).