FIGURE 5.

Double-shelled ellipsoidal solute model. This model (Asami et al., 1980) was used to analyze the dielectric spectrum of F-actin solution as outlined in Materials and Methods. The expressions 2r_x and 2r_y refer to the lengths of major and minor axes of the ellipsoidal shells, respectively. Theoretical spectral curves obtained were superimposed on the observed values for Δɛ′(f) and Δɛ″(f) (blue-colored solid lines in Fig. 3 a). Note that when applied to the spectrum of the myoglobin solution with the protein axial ratio of 1.001, a curve essentially the same as estimated by the previous method was obtained (Yokoyama et al., 2001).