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. 2003 Nov;85(5):3255–3261. doi: 10.1016/S0006-3495(03)74743-2

FIGURE 3.

FIGURE 3

Intrinsic fluorescence anisotropy measurements of GR. Samples (0.54 μM GR) were incubated for 90 min at room temperature with the indicated concentrations of GdnHCl and fluorescence anisotropy was measured as described in Materials and Methods. Excitation was at 285 nm and emission was measured at 315 nm. Symbols correspond to means ± standard deviations of at least six independent measurements at each GdnHCl concentration.