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Size-exclusion HPLC analysis of GR. From top to bottom, GR samples were incubated for 90 min in the absence or in the presence of 1, 2.5, and 3 M GdnHCl before injection into the column. The HPLC elution buffer did not contain GdnHCl (see Materials and Methods). Apparent molecular weights of each peak obtained from the calibrated column are indicated.
