FIGURE 2.

Temperature effects on unfolding length and percentage of tandem events. (A) Peak-to-peak unfolding length from ensembles of extension curves at 10°C and 42°C. The unfolding distributions for both temperatures were fitted with sums of Gaussians that reflect proportional contour lengths for single repeats. The minor peaks were likewise fitted but with proportional contour lengths of tandem repeats. The overall sum of all the Gaussians is indicated by the heavy black line. (B) A factor of nearly two between the major (single repeat) and minor (tandem repeats) peaks is apparent for temperatures from 10 to 42°C. (C) The percentage of tandem repeat unfolding events decreases with temperature. The dotted arrow indicates that in cooling from 42°C to 23°C, the percentage of tandem repeat unfolding events returns to within 90% of the initial value.