FIGURE 2.

(A) Visualization of phase separation (liquid-disordered and gel-phase) for the binary DSPC/DOPC (0.5/0.5 molar ratio) mixture (0.1 mol % DiI-C₁₈): the fluorescence image represents a three-dimensional projection of a GUV reconstructed from confocal slices (∼0.4-μm-thick) with the Zeiss software of ConfoCor2. (B) three-dimensional projection of a GUV (DOPC/DSPC 0.5/0.5 molar ratio and 10 mol % of cholesterol, with 0.1 mol % DiI-C₁₈) reconstructed from confocal slices as described in A. The homogeneous fluorescence corresponds to a single fluid phase. (C) Phase separation as visualized in GUV prepared from the ternary mixture DOPC/DSPC (0.5/0.5 molar ratio) and 20 mol % of cholesterol (0.1 mol % DiI-C₁₈): the fluorescence image represents a three-dimensional projection of a GUV reconstructed from confocal slices as described in A. (D) Phase separation as visualized in GUV prepared from the ternary mixture DOPC/DSPC (0.5/0.5 molar ratio) and 33 mol % of cholesterol (0.1 mol % DiI-C₁₈): the fluorescence image represents a three-dimensional projection of a GUV reconstructed from confocal slices as described in A.