FIGURE 3.

(A) FCS autocorrelation curves are shown for DiI-C₁₈ mobility in DPPC/cholesterol GUVs, for 33 mol % of cholesterol (solid), for 50 mol % (dashed), and for 67 mol % (dot). (B) FCS autocorrelation curves are shown for DiI-C₁₈ mobility in DSPC/cholesterol GUVs, for 33 mol % of cholesterol (solid), for 50 mol % (dashed), and for 67 mol % (dot). (C) Average diffusion coefficients of DiI-C₁₈ in DPPC/cholesterol (solid squares), in DSPC/cholesterol (unfilled squares), and sphingomyelin/cholesterol (triangles) GUVs, as determined from the fitting of the autocorrelation curves, are reported as a function of cholesterol concentration. Bars represent the standard deviation from the average values (see Materials and Methods for details).