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Plot of ANS fluorescence intensity in arbitrary units, versus alcohol concentration (a). The protein and dye concentration were 25 μM. The excitation wavelength is 370 nm. The dotted lines correspond to the derivate dβ/da, and the maxima to the inflexion points of compressibility curves: HFIP (–·–·–), TFE(- - - -), and IPA (·····).
