TABLE 2.

Thermodynamic parameters of the binding of custom oligonucleotides, 20-, and 30-basepairs long (see Materials and Methods), to EDOPC as determined by fitting of the experimental binding data to Eq. 7

		DNA concentration, μM
Lipid:DNA charge ratio		1, 20 bp	3, 20 bp	3, 30 bp
2:1	ΔG150	−280 ± 7	−338 ± 18	−203 ± 5
	m	0.37 ± 0.04	0.94 ± 0.15	0.21 ± 0.02
	[NaCl]cr	906 ± 63	510 ± 40	1109 ± 66
4:1	ΔG150	−380 ± 21	−374 ± 11	−172 ± 5
	m	1.07 ± 0.15	0.92 ± 0.07	0.43 ± 0.07
	[NaCl]cr	504 ± 30	555 ± 19	553 ± 54
8:1	ΔG150	−325 ± 19	−334 ± 20	−257 ± 19
	m	0.52 ± 0.09	0.69 ± 0.10	0.88 ± 0.14
	[NaCl]cr	771 ± 73	633 ± 45	440 ± 26
16:1	ΔG150	−448 ± 34	−377 ± 14	−337 ± 58
	m	0.99 ± 0.14	0.52 ± 0.04	0.58 ± 0.18
	[NaCl]cr	604 ± 29	879 ± 33	731 ± 82

Column titles specify DNA concentration and oligonucleotide length, in basepairs (bp).