FIGURE 5.

Kinetics of tethering of CHO cells expressing WT or mutant GPIb to vWF-A1 surfaces: Initial rate of tethering of CHO cells expressing WT or Q232V or K237V mutants (A) to surfaces coated with 100 μg/ml vWF-A1 at the indicated shear stresses and (B) at a shear stress of 1.2 dyn/cm² on surfaces coated with different concentrations of vWF-A1. (C) The distance traveled by tethered cells at 1.6 dyn/cm² shear stress before detachment from surfaces coated with 100 μg/ml vWF-A1. The cell diameter is ∼15 μm. (D) The fraction of the cells that are transiently tethered before detachment or converted to rolling adhesion. The data presented are means ± SD of three to five experiments.