FIG. 2.

Colonization deficiency of LPS and CPS mutants. Each point represents the value from a single mouse competition assay. The competitive index is defined as the output ratio of mutant to wild-type bacteria divided by the input ratio of mutant to wild-type bacteria. Strains with the wild-type ability to colonize have a competitive index of approximately 1.0. Data sets: 1, MO10 wbfF; 2, MO10 ΔgalEK; 3, MO10 ΔgalEK pACYCgalE; 4, MO10 ΔwaaL; 5, MO10 ΔwaaL pBADwaaL; 6, MO10 wavB; 7, MO10 wavB, pSSkanwavB2; 8, MO10 waaF; 9, MO10 waaF, pACYCwaaF; 10, MO10 ΔwaaL ΔwbfF. The defect in colonization of all mutant strains was statistically significant (P < 0.01) compared with the wild-type strain MO10 by Student's two-tailed t test.