TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotypea | Reference or source |
|---|---|---|
| Strains | ||
| MO10 | Clinical isolate of V. cholerae O139 | 42 |
| PW96 | MO10 lacZ::MCS | 43 |
| MO10 ΔwaaL | MO10 ΔwaaL | This study |
| MO10 ΔwaaLwbfF | MO10 ΔwaaL wbfF::pGP704 | This study |
| MO10 ΔgalEK | ΔgalE::cm ΔgalK | This study |
| MO10 wbfF | wbfF::pGP704 | This study |
| MO10 waaF | waaF::pGP704 | This study |
| MO10 wavB | wavB::pGP704 | This study |
| Plasmids | ||
| pTrcAkan | Cloning vector Kmr | 24 |
| pBAD28 | Cloning vector Apr Kanr | 8 |
| pGP704 | OriR6K mobRP4 Apr | 22 |
| pUC4K | Apr Kmr | 39 |
| pGPwaaF | ‘waaF’b Apr | 25 |
| pGPwavB | ‘wavB’ Apr | 25 |
| pKEK229 | OriR6K mobRP4 sacB Apr | 6 |
| pKEKΔgalE::cm | ΔgalE::cm Apr | 26 |
| pBADwaaL | waaL+ Kmr | 25 |
| pACYCgalE | galE+ Kmr | 26 |
| pACYCwaaF | waaF+ Kmr | 25 |
| pAKwavB | wavB tetR+ Cmr | 25 |
| pGPwbfF | ‘wbfF’ in pGP704 Apr | This study |
| pKEKΔgalK | ΔgalK in pKEK229 Apr | This study |
| pKEKΔwaaL | ΔwaaL in pKEK229 Apr | This study |
| pSSkanwavB1 | pAKwavB tetR+ Cmr Kmr | This study |
| pSSkanwavB2 | pSSkanwavB1 ΔtetR Cmr Kmr | This study |
a
MCS, multiple cloning site.
b
‘gene,’ internal gene fragment.