Table 2. Proteins identified in the conditioned medium of a 5-day mouse testicular cell culture based on the Mus musculus database.
| Name of Protein | Number of Accessions | Number of unique peptides | Score sequest (HT > 0) | Location subcellular | Function |
|---|---|---|---|---|---|
| The Extracellular matrix: | |||||
| Collagen alpha-1(I) chain (COL1A1) | P11,087-1 | 34 | 193 | Extracellular matrix | Structure, extracellular matrix, binding |
| Collagen alpha-2(I) chain COL1A2 | Q01,149 | 29 | 198 | Extracellular matrix | Structure, extracellular matrix, binding |
| Collagen alpha-2(V) chain (COL5A2) | Q3U962 | 3 | 15 | Extracellular matrix | A key determinant in the assembly of tissue-specific matrices |
| Collagen alpha-1(III) chain (COL3A1) | P08,121 | 3 | 14 | Extracellular matrix | Extracellular matrix |
| Fibronectin (FN1) | P11,276 | 11 | 43.8 | Extracellular matrix | Cell Adhesion, signaling-receptor binding, |
| Biglycan (BGN) | P28,653 | 3 | 17 | Extracellular matrix | Collagen fiber assembly, extracellular matrix binding |
| Fibrillin-1 (FBN1) | Q61,554 | 3 | 12 | Extracellular matrix | Components of extracellular matrix structure |
| Latent-transforming growth factor beta-binding protein 2 (LTBP2) | O08,999 | 2 | 5 | Extracellular matrix | Plays a role in the organization of fiber-elastic structures |
| Actin, alpha cardiac muscle 1 (ACTC1) | P68,033 | 2 | 33 | Extracellular matrix | Actin-filament organization |
| Cell Structure: | |||||
| Collagen alpha-1(IV) chain (COL4A1) | P02,463 | 11 | 77 | Basement membrane | the major structural component of basement membranes |
| Collagen alpha-2(IV) chain (Col4a2) | P08,122 | 2 | 8 | Basement membrane | the major structural component of basement membranes |
| SPARC (SPARC) | P07,214 | 11 | 101 | Basement membrane | regulate cell growth through interactions with the extracellular matrix and cytokines. |
| Basement membrane-specific heparan sulphate proteoglycan core protein (HSPG2) | Q05,793 | 2 | 7 | Basement membrane | Component of the basement membrane |
| Cathepsin L1 (CTSL) | P06,797 | 3 | 5 | Basement membrane, Lysosome | Thiol protease important for the overall degradation of proteins in lysosomes |
| Actin, cytoplasmic 1 (ACTB) | P60,710 | 7 | 82.3 | Cytoskeleton, Nucleus | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Regulate gene transcription and motility and repair of damaged DNA |
| 72 kDa type IV collagenase (MMP2) | P33,434-1 | 2 | 6 | extracellular space, mitochondrion, nucleus, plasma membrane | Various functions include vascular remodeling, angiogenesis, tissue repair, tumors invasion, and inflammation. |
| Nucleoside diphosphate kinase A (NME1) | P15,532 | 2 | 4 | Nucleus, cytoplasm | Key role in the synthesis of nucleoside triphosphates other than ATP |
| Secretion: | |||||
| Plasminogen activator inhibitor 1 (SERPINE1) | P22,777 | 17 | 127 | Secretion | Protease binding, Signalling-receptor binding, Serine protease inhibitor |
| Clusterin (CLU) | Q06,890 | 12 | 45 | Secreted, nucleus, cytoplasmic, cytosol, mitochondria | Binding regulation the cell proliferation |
| Insulin-like growth factor-binding protein 7 (IGFBP7) | Q61,581 | 8 | 39 | Secreted | Binds IGF-I and IGF-II with a relatively low affinity Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion |
| Follistatin-related protein 1 (FSTL1) | Q62,356 | 8 | 4 | Secreted | Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation |
| Metalloproteinase inhibitor 1 (TIMP1) | P12,032 | 7 | 29.7 | Secreted | Growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling |
| Pigment epithelium-derived factor (SERPINF1) | P97,298 | 6 | 26.3 | Secreted, melanosome | Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. |
| Cystatin-C (CST3) | P21,460 | 5 | 34 | Secreted | This protein is thought to serve an important physiological role as a local regulator of this enzyme activity |
| Procollagen C-endopeptidase enhancer 1 (PCOLCE) | Q61,398 | 4 | 13 | Secreted | Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. |
| Prosaposin (PSAP) | Q61,207 | 2 | 8 | Secreted, Lysosome | Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling |