Figure 3.

Behavioral phenotype of mice treated with anti-NMDAR and control antibodies during week 1 of antibody washout. Following the habituation in the testing room environment, mice were allowed to explore the test arena; the total distance traveled and time spent in the center of the arena were recorded. Mice treated with anti-NMDAR antibodies did not differ in their motor or anxiety phenotypes in an open field test (A-B). In the object location paradigm, the acquisition trial during which mice were presented with two identical objects was followed by a retention period and a test trial during which one object was displaced to a new location (NL) diagonally from the object in the familiar location (FL). The time spent in the NL and FL were recorded and the NL index was calculated as the ratio of NL and the total time spent in NL and FL. In a Y maze paradigm, mice were allowed to explore three open arms of the Y maze; the alternation index was calculated as a ratio of the number of alternations to the number of choices. Mice treated with anti-NMDAR developed impaired spatial discrimination in the object location paradigm (C) while their memory was intact in a Y-maze paradigm (D). *, p < 0.05, t-test. The data represent mean ± SEM; n = 9–11 per group; NL, new location.