Figure 7.

Assessment of neurogenesis after 4 weeks of antibody washout. Mice treated with anti-NMDAR antibodies and control antibodies displayed similar patterns of total BrdU immunoreactivities in the granular zone (GZ) and subgranular zone (SGZ) as well as a molecular layer (ML) and dentate hilus (DH) (A). Mice treated with anti-NMDAR antibodies demonstrated a higher bright-to-faint BrdU+ cell ratio (B) and lower densities of doublecortin-positive (DCX+) cells in the hippocampal GZ and SGZ compared to the mice treated with control antibodies after 4 weeks of antibody washout (C). The data represent mean ± SEM. *, p < 0.05, ***, p < 0.001, t-tests; n = 6 mice per group. Representative microscopic images showing the DCX and BrdU immunoreactivities in the hippocampus of mice treated with control antibodies (top panel) and anti-NMDAR antibodies (lower panel) (D). Scale bar: 117 μm.