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Lateral diffusion of a single RyR2 channel labeled directly with Alexa-488. (Left panel) An x,y fluorescence image of a single RyR2 (indicated by the arrow). (Right panel) A random walk trace for this RyR2 channel (recorded at 3-s intervals in the absence of annexin 12). The results are similar to those obtained when fluo-3 fluorescence was used to track RyR2 position and for RyR2D4365-GFP.
