FIGURE 3.

Cumulative data showing effects of annexin 12 on the lateral diffusion of single RyR2 channels in bilayers. Data in these figures document further the ability of annexin 12 to reduce RyR2 diffusion in bilayers. The relationships between annexin-12 concentration and either the apparent lateral diffusion coefficient (D_RyR) calculated for single RyR2 channels (left panel) or the mean distance moved by RyR2 during the 3-s imaging interval (right panel) are shown. Addition of PS to the bilayer in the absence of annexin-12 did not affect RyR2 diffusion. Results are means and SD of six determinations.