Temperature dependence of the reaction enthalpy, ΔH0, and the binding constant, K0, for TAT-PTD binding to heparan sulfate. (A) versus temperature. (B) K0 versus temperature. (⋄) Experimental values. Solid line in A, linear regression analysis yielding (kcal/mol) = 0.135 T(C°) − 8.108. Solid line in B, predicted temperature dependence of K0 using the above regression formula for ΔH0(T).