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. 2004 Jan;86(1):296–307. doi: 10.1016/S0006-3495(04)74105-3

FIGURE 1.

FIGURE 1

Apical and basolateral membranes of cultured epithelial cells showed altered sensitivity to lysenin. (A–F) Binding of lysenin to MDCK II cells grown on coverslips. MDCK II cells at low density (A and D) or at high density (B, C, E and F) were stained with lysenin as described under Experimental Procedures. Lysenin staining (A–C) and DIC micrographs (D–F) of the same specimens are shown. Bar, 10 μm. (G) Lysenin was added to filter-grown MDCK II cells from apical side (solid column) or basolateral side (hatched column). The cells were incubated with lysenin for 30 min at 37°C. Viability was measured as described in Experimental Procedures.