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. 2004 Jan;86(1):296–307. doi: 10.1016/S0006-3495(04)74105-3

FIGURE 2.

FIGURE 2

Glycolipid content affects lysenin sensitivity in mouse melanoma cells. (A–D) Immnunofluorescence staining of mouse melanoma cells with lysenin. MEB4 (A and C) and GM95 (B and D) were stained with lysenin as described under Experimental Procedures. Lysenin staining (A and B) and DIC micrographs (C and D) of the same specimens are shown. Bar, 10 μm. (E) Cytolytic sensitivity of MEB4 (filled bar), GM95 (hatched bar), or CG1 (open bar) to lysenin. Cells were incubated with various concentrations of lysenin for 30 min at 37°C. Viability was measured as described in Experimental Procedures.