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. 2004 Jan;86(1):296–307. doi: 10.1016/S0006-3495(04)74105-3

FIGURE 5.

FIGURE 5

Local density of SM influenced the binding of lysenin to SM in lipid bilayers. (A–C) GUVs composed of C16:0 SM/diC18:1 PC (molar ratio 7:3) containing 7 mol % diC16:0 PG and 3 mol % diC12:0 PG (A and B, two representative fields were shown) and C16:0 SM/diC16:0 PC (molar ratio 1:1) containing 10 mol % diC 16:0 PG (C) labeled with 0.1% DiI C18 (red) and 0.1% BODIPY-C12-PC (green) were prepared as described under Experimental Procedures. Color merged images were shown. Bar, 2 μm. (D) Binding of lysenin to egg SM/diC18:1 PC (molar ratio 3:7), egg SM/diC16:0 PC (3:7), or egg SM/GalCer (3:7) liposomes. The binding was measured as described in Experimental Procedures. The data are representative of two independent experiments yielding similar results. (E and F) GUVs of C16:0 SM/diC18:1 PC (3:7) (E) or C16:0 SM/diC16:0 PC (3:7) (F) were incubated with His-Venus-lysenin as described in Experimental Procedures. His-Venus-lysenin fluorescence images were obtained with confocal microscope. Bar, 5 μm.