TABLE 2.

EPR accessibility and depth parameters

Labeling site	Π(O2)*	Π(NiEDDA)†	Φ‡	Depth (Å)‡
C4	0.48 ± 0.02	0.14 ± 0.02	1.23 ± 0.20	9.5 ± 1.0
C8	0.60 ± 0.02	0.10 ± 0.03	1.79 ± 0.39	12.2 ± 1.8
C10	0.42 ± 0.02	1.48 ± 0.02	−1.26 ± 0.06	−1.9 ± 1.0
C11	0.51 ± 0.03	0.48 ± 0.02	0.06 ± 0.10	4.2 ± 0.4
C12	0.64 ± 0.01	0.10 ± 0.02	1.86 ± 0.22	12.5 ± 0.8
C14	0.45 ± 0.02	0.84 ± 0.02	−0.62 ± 0.06	1.0 ± 0.4

^*Accessibility parameter for air (20% O₂).

^†Accessibility parameter for 20 mM NiEDDA. Uncertainties in Π values are standard errors based on replicate experiments (n = 2–3 for each site).

^‡Uncertainties in Φ and depth are calculated from the maximum uncertainties in Π values.