TABLE 3.
Partition coefficients for the binding of MTSL-labeled CM15 analogs with PE/PG and bacterial polar lipid vesicles
| Kp (M−1) | ||
|---|---|---|
| Labeling site | POPE/POPG (8:2) | BPL |
| C4 | 0.04 ± 0.02 × 104 | 0.46 ± 0.15 × 104 |
| C8 | 0.39 ± 0.04 × 104 | 1.78 ± 0.35 × 104 |
| C10 | 0.31 ± 0.04 × 104 | 0.95 ± 0.31 × 104 |
| C11 | 0.30 ± 0.03 × 104 | 0.83 ± 0.26 × 104 |
| C12 | 0.40 ± 0.04 × 104 | 1.81 ± 0.32 × 104 |
| C14 | 0.33 ± 0.03 × 104 | 1.53 ± 0.33 × 104 |
Kp was determined from the maximum slope of binding isotherms as shown in Fig. 5. Error estimates are based on the uncertainty in the linear regression analysis. Replicate experiments were in good agreement with the values shown (i.e., within the given error limits). The composition of BPL is PE/PG/cardiolipin (68:26:6 mol %).