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. 2004 Jan;86(1):488–498. doi: 10.1016/S0006-3495(04)74127-2

FIGURE 4.

FIGURE 4

Transient expression of FV proteins in serum-free culture medium COS-1 cells were transiently transfected to express WT-FV or the FV mutants. FV antigen levels were measured in the culture media by ELISA. Bars represent the means ± standard deviations (n = 6). The mean value of WT-FV was 23.8 ng/35 mm well and is assigned to be 100% in this figure. This WT-FV construct was used as a template for the D/G mutation and to construct the WT* plasmid (which only expresses the FV2 isoform). There was no significant difference between the expression levels of the WT FV-plasmid and the WT* plasmid, the WT* plasmid serving as a template for the other FV mutants (nomenclature: D2194G (D/G), D2194K (D/K), K2101E/K2103E (KK/EE), K2101E/K2103E/D2194K (DKK/KEE), and C2038A/C2193A (CC/AA)).