TABLE 4.

Relative change in activity at concentrations around induction ([CI_t] ≈ [Cro_t] ≈ 24 nM) at P_RM and P_R corresponding to perturbations as performed in Table 3

	+1 kcal/mol		−1 kcal/mol
	PRM	PR	PRM	PR
ΔG1	−0.5	1.2	0.3	−0.7
ΔG2	−0.5	1.0	0.4	−0.7
ΔG3	0	0	0	0
ΔG12	−0.5	0.9	0.4	−0.7
ΔG23	0	0	0	0
ΔG1′	0	0	0	0
ΔG2′	0	0	−0.1	−0.1
ΔG3′	0.6	0	−0.6	0
ΔG12′	0	0	0	0
ΔG23′	0	0	−0.1	−0.1
ΔG123′	0	0	0	0
ΔGRM	−0.7	0	0.8	0
ΔGR	0.3	−0.7	−0.5	1.4

Wild-type activity in regime 2 is 3.0 × 10⁻³ s⁻¹ at P_RM and 4.0 × 10⁻³ s⁻¹ at P_R.