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. 2004 Jan;86(1):629–645. doi: 10.1016/S0006-3495(04)74141-7

FIGURE 3.

FIGURE 3

Proposed mechanisms and products of NADH photolysis in the presence of O2 or oxidized cytoc. (A) Proposed reaction pathways for oxygen or oxidized cytoc for scavenging photolysis-generated free radicals. (B) Effect of NADH photolysis on hemoglobin (Hb) oxygenation. A series of UV pulses was applied to a 200-μM NADH sample including 250 μM of hemoglobin. The absorption spectrum of the hemoglobin is presented in a stack plot. The oxygenation of the sample decreased with each irradiation pulse as evidenced by the decrease in absorbance at ∼560 nm. Note that the nonlinear dependence of hemoglobin oxygenation on oxygen tension contributed to the nonlinear response of absorbance to the photolysis steps. (C) Reduction of cytoc during the photolysis of NADH. A series of UV pulse as applied to the sample of NADH including 20 mM of oxidized cytoc. An increase in cytoc reduction was observed by the absorbance changes at ∼550 nm. Absorbance changes at ∼520 and ∼416 nm were also characteristic of cytoc.