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Effect of pre-NADH photolysis on mitochondria extract DH enzymatic activity. The production NADH rate was monitored on extracts after preirradiated intact mitochondria with different UV power levels from 18.6 to 80 mW. Extract reaction was initiated by the addition of 5 mM G/M along with varying amounts of NAD. NADH levels were monitored spectrophotometrically in a standard cuvette instrument (PerkinElmer).
