FIGURE 7.

Time course of NADH fluorescence in an ED-FRAP experiment. Experiment was performed on the HDH system outlined in Methods. Analysis focused on the initial rate of recovery that was linear for the first 1-s measurement. Different levels of NADH photolysis (shown in small panel) essentially simultaneously varied the [NADH] and [NAD] aiding in the extraction of kinetic parameters for the reaction. Note that in this specific case, the full recovery can be considered as a simple monoexponential process.