FIGURE 8.

Initial slope analysis of GDH NADH ED-FRAP experiments. (A) Linear regression of initial NADH recovery after photobleaching for different levels of NADH photolysis. Note the increase of the slope when the amount of [NADH] is decreased and [NAD] is increased. (B) Linear relationship between initial slope and initial [NADH₀] level. To extrapolate to [NADH₀] = 0 allows obtaining an estimation of V_maxf; see text. (C) Estimated V_maxf vs. [GDH] concentration. The linear relationship shows that the estimated V_maxf is well related to enzyme concentration.