FIGURE 9.

Initial slope analysis of ADH NADH ED-FRAP experiments. (Top left) Relation between initial slope and amount of photolyzed NADH. A classical Michaelis-Menten relationship is observed as expected. (Top right) Linear relationship observed between the parameters 1/C₁ and [NADH] consistent with Eq. 7. (Bottom left) Effect of [ADH] variation on the relation between the initial recovery and the amount of photolyzed NADH. (Bottom right) Demonstration of the dependence of the parameter C₁ of Eq. 7 with [ADH].