TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Reference or source
E. coli XL-1 Blue	recA1	Stratagene
S. pyogenes
90-226	M1 serotype	16
90-226 emm1::Km	emm1::Km	16
DC276	emm1::Km, fba::pFW5	This study
DC283	fba::pFW5	This study
DC294	DC276 (pYT1143)	This study
DC297	DC283 (pYT1143)	This study
Plasmids
pFW5	GAS suicide vector	42
pYT1143	Plasmid for expression of fba in GAS	48
pSportI	Cloning vector	Invitrogen
pSport-fba	fba gene cloned into pSportI	This study
pFW5Δfba	730-bp internal fragment of fba cloned into pFW5	This study