Domain structure of T. scotodustus DNA ligase. (A) T. scotoductus DNA ligase (Tslig) is illustrated as a linear assortment of conserved structural domains (Ia, Adenylation, OB (Oligomer Binding) fold, Zn-binding, HhH (Helix-hairpin-Helix), and BRCT (BRCA1 C-terminus)). D1 to D4, domain 1 to domain 4. (B) Schematic view of the three-dimensional structure of Tslig. The tryptophan (W) and tyrosine (Y) residues are rendered as white and gray space filled structures, respectively. Tslig contains 6 W and 21 Y. The covalently bound AMP moiety is pointed out by an arrow.