TABLE 1.
Thermodynamic parameters of heat-induced unfolding of deadenylated and adenylated Tslig Parameters are determined from data shown in Fig. 2
Tmax* (°C) | Transitions (n)† | Tm‡ (°C) | ΔHcal (kJ mol−1) | Σ ΔHcal (kJ mol−1) | |
---|---|---|---|---|---|
Deadenylated Tslig | 89.0 | 2 | 88.9 | 904 | 1649 |
96.6 | 96.5 | 745 | |||
Adenylated Tslig | 95.2 | 3 | 91.8 | 301 | 1729 |
101.3 | 95.6 | 1009 | |||
101.4 | 417 |
Tmax corresponds to the temperature at the top of the peak.
n refers to the number of calorimetric domains identified by deconvolution of DSC thermograms.
Tm is the melting point of the unfolding transition.