TABLE 2.
Lifetimes and amplitudes relative to the phosphorescence decay in buffer at 20°C of apo- and Cd-azurins
| Protein | τ1 (s) | α1 | τ2 (s) | τav (s) | ητav(cp) | ΔH#(1/ητav) (kcal mol−1) |
|---|---|---|---|---|---|---|
| Apo-WT | 0.63 ± 0.02 | 1.0 | – | 0.63 ± 0.02 | 5.0 × 104 | 24 ± 1.5 |
| Apo-F110S | 1.49 ± 0.05 | 0.48 ± 0.02 | 0.81 ± 0.03 | 1.14 ± 0.05 | 1.6 × 105 | 38 ± 2.0 |
| Apo-I7S | 0.18 ± 0.01 | 0.81 ± 0.01 | 0.06 ± 0.003 | 0.16 ± 0.01 | 3.7 × 103 | 22 ± 1.0 |
| Cd-WT | 0.55 ± 0.01 | 1.0 | – | 0.55 ± 0.01 | 3.8 × 104 | 24 ± 1.5 |
| Cd-F110S | 3.36 ± 0.10 | 1.0 | – | 3.36 ± 0.10 | 2.3 × 107 | 33 ± 1.5 |
| Cd-I7S | 0.41 ± 0.02 | 0.17 ± 0.01 | 0.26 ± 0.01 | 0.25 ± 0.02 | 7.7 × 103 | 21 ± 1.0 |
η(τav) has been calculated according to Gonnelli and Strambini (1995). ΔH#(ητav) are the activation enthalpies obtained from the temperature dependence of 1/η(τav).