Skip to main content
. 2004 Feb;86(2):1209–1222. doi: 10.1016/S0006-3495(04)74195-8

FIGURE 1.

FIGURE 1

Substrate patterning for growing isolated myotubes. (A) A glass slide is first spin coated with photoresist (1), and then exposed to ultraviolet light through a mask of the desired pattern (2). The exposed resist is then developed away, resulting in lithographically patterned slides (3), and the bare regions of glass are finally coated with ATC (4). After the patterned photoresist is stripped away, pAAm is bound to the silane-coated glass (5) and mPEG is then bound to the pAAm gel (6), resulting in p(AAm-co-EG) IPN patterned slides (7). (B) The p(AAm-co-EG) IPN patterned slides are finally treated with APES and collagen. After 24 h, fluorescently labeled collagen I preferentially adsorbs to the amino-silane coated glass and not to the IPN.