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. 2004 Feb;86(2):1234–1242. doi: 10.1016/S0006-3495(04)74197-1

FIGURE 1.

FIGURE 1

Polybrene's enhancement of retrovirus transduction and adsorption is saturable, and is not due to toxicity or limiting concentrations of virus. (A) Ecotropic virus was supplemented with concentrations of polybrene between 0 and 28 μg/ml. NIH-3T3 cells were exposed to the virus samples for 48 h, lysed, and β-galactosidase levels were measured using an established ONPG-based assay. (B) An ecotropic virus stock (solid circles), as well as 1:2 (open circles) and 1:4 (solid squares) dilutions of the stock in fresh medium, were supplemented with between 0 and 12 μg/ml polybrene. Confluent NIH-3T3 cells were exposed to the same virus samples for 2 h, lysed, and levels of cell-associated p30 were measured via ELISA. (C) NIH-3T3 cells were plated in a 96-well plate at 5000 cells/well. After 24 h, the medium was removed and then replaced with fresh medium with concentrations of polybrene between 0 and 64 μg/ml. After 48 h, the relative cell density was measured via Orange G assay.