Skip to main content
. 2004 Feb;86(2):1234–1242. doi: 10.1016/S0006-3495(04)74197-1

FIGURE 4.

FIGURE 4

Adsorption of FITC-labeled poly-L-lysine on target cells is rapid, dose dependent, and independent of cell type. (A) Confluent NIH-3T3 cells were trypsinized and pelleted via centrifugation for 5 min at 100 × g, 4°C. The cell pellets were resuspended in concentrations of FITC-labeled, 50-kDa mean molecular mass poly-L-lysine between 0 and 100 μg/ml, then incubated at 37°C on a rotary shaker. After 1 h, the cells were pelleted, washed with 1% FBS/PBS, resuspended in ice-cold 1% FBS/PBS, and analyzed via flow cytometry. (B) NIH-3T3 cells prepared as described above were exposed to 40 μg/ml 50-kDa mean molecular mass PLL for various times then analyzed via flow cytometry. (C) Confluent NIH-3T3 (solid circles) and CHO (open circles) cells prepared as described above were exposed to various concentrations of 50-kDa mean molecular mass poly-L-lysine for 1 h, then analyzed via flow cytometry.