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Interaction energy of a permeating Cl− with the various constituents of (a) the ClC channel and (b) the pore region, calculated using a cutoff distance of 16 Å for nonbonded interactions. The standard deviation for the energy is ±5 kcal/mol for pore-lining residues, ±15 kcal/mol for water, and ±2 kcal/mol for bulk protein.
