FIG. 2.
Association of PMT with vimentin. (A) Purified mouse vimentin (1.5 μg) was subjected to the ligand overlay assay with PMT. (B) Pull-down assay with GST-PMT840-985. Vimentin (2 μg) was mixed with GST-PMT840-985- or GST-coupled glutathione Sepharose 4B and incubated at 4°C for 3 h. Vimentin associated with the beads was extracted by boiling in SDS-PAGE buffer and subjected to Western blot analysis with anti-vimentin antibody as described in the text. The positions of the molecular mass standards are shown on the left.